Journal: RSC Advances

Article Title: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface †

doi: 10.1039/c9ra00761j

Figure Lengend Snippet: Concentration of focal adhesion proteins, vinculin (A), talin (B), measured by the enzyme-linked immunosorbent assay (ELISA) in human Saos-2 osteoblast-like cells cultured 24 hours on untreated cpTi, differently nanostructured cpTi by anodic oxidation at 10 V, 15 V, 20 V and control glass. P values < 0.05 (marked by group name) and P < 0.01 (marked by group name and an asterisk) considered significant versus samples labelled above.

Article Snippet: Saos-2 cells (passage 88, CLS Cell Lines Service GmbH, Germany) were seeded on cpTi scaffolds at the density 1.5 × 10 4 cells per cm −2 .

Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Control

Journal: RSC Advances

Article Title: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface †

doi: 10.1039/c9ra00761j

Figure Lengend Snippet: Immunofluorescence staining of vinculin (A–F) and talin (G–L) in human Saos-2 osteoblast-like cells cultured on cpTi samples 48 h after seeding. Untreated cpTi (A and G), nanostructured cpTi by anodic oxidation at 10 V (B and H), 15 V (C and I), 20 V (D and J) and control glass (E and K). Separated channels in photomicrographs of 15 V sample (F and L). Vinculin and talin (green), F-actin (red) and nucleus (blue). Objective 63× , magnification 2× , scale 20 μm, immerse oil.

Article Snippet: Saos-2 cells (passage 88, CLS Cell Lines Service GmbH, Germany) were seeded on cpTi scaffolds at the density 1.5 × 10 4 cells per cm −2 .

Techniques: Immunofluorescence, Staining, Cell Culture, Control

Journal: RSC Advances

Article Title: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface †

doi: 10.1039/c9ra00761j

Figure Lengend Snippet: Visualization of human Saos-2 osteoblast-like cells adhered on untreated and nanostructured cpTi scaffolds 48 hours after seeding, scanning electron microscopy. Cells cultured on untreated cpTi (A), nanostructured cpTi by anodic oxidation at 10 V (B), 15 V (C), 20 V (D) and control glass (E). Magnification 2k×. The marked squares of SEM photographs of the upper row are magnified in a lower row.

Article Snippet: Saos-2 cells (passage 88, CLS Cell Lines Service GmbH, Germany) were seeded on cpTi scaffolds at the density 1.5 × 10 4 cells per cm −2 .

Techniques: Electron Microscopy, Cell Culture, Control

Journal: RSC Advances

Article Title: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface †

doi: 10.1039/c9ra00761j

Figure Lengend Snippet: Proliferation (A), metabolic activity (B) and osteogenic differentiation (C–E) of human Saos-2 osteoblast-like cells cultured on untreated and differently nanostructured cpTi samples. Untreated cpTi (Ti), differently nanostructured cpTi samples by anodic oxidation at 10 V, 15 V, 20 V and control glass. Day (D), no significant differences were observed in the cell proliferation. (B) P values < 0.05 (marked by group name) considered significant versus samples labelled above columns. (C–E) Relative expression of RunX2 mRNA (C), relative expression of osteocalcin mRNA (D), relative expression of osteocalcin mRNA (E) P values < 0.01 (marked by group name) and <0.005 (marked as name of the group with a star) considered significant versus samples labelled above columns.

Article Snippet: Saos-2 cells (passage 88, CLS Cell Lines Service GmbH, Germany) were seeded on cpTi scaffolds at the density 1.5 × 10 4 cells per cm −2 .

Techniques: Activity Assay, Cell Culture, Control, Expressing

Journal: RSC Advances

Article Title: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface †

doi: 10.1039/c9ra00761j

Figure Lengend Snippet: Alkaline phosphatase activity of human Saos-2 osteoblast-like cells cultured on untreated and differently nanostructured cpTi samples. Untreated cpTi (Ti), differently nanostructured cpTi samples by anodic oxidation at 10 V, 15 V, 20 V and control glass. P values < 0.05 (marked by group name) and <0.01 (marked as name of the group with a star) considered significant versus samples labelled above.

Article Snippet: Saos-2 cells (passage 88, CLS Cell Lines Service GmbH, Germany) were seeded on cpTi scaffolds at the density 1.5 × 10 4 cells per cm −2 .

Techniques: Activity Assay, Cell Culture, Control

Journal: RSC Advances

Article Title: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface †

doi: 10.1039/c9ra00761j

Figure Lengend Snippet: Detection of collagen type I (A and B) and osteocalcin (C) in human Saos-2 osteoblast-like cells cultured on untreated cpTi (Ti), differently nanostructured cpTi scaffolds by anodic oxidation at 10 V, 15 V, 20 V and control glass (G). Data shown as collagen type I and osteocalcin fluorescence intensity calculated from photomicrographs. Day (D), P values < 0.05 (marked by group name) considered significant versus samples labelled above columns.

Article Snippet: Saos-2 cells (passage 88, CLS Cell Lines Service GmbH, Germany) were seeded on cpTi scaffolds at the density 1.5 × 10 4 cells per cm −2 .

Techniques: Cell Culture, Control, Fluorescence

Journal: RSC Advances

Article Title: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface †

doi: 10.1039/c9ra00761j

Figure Lengend Snippet: Immunohistochemical staining of collagen type I in Saos-2 cells cultured on untreated cpTi and differently nanostructured cpTi scaffolds on day 7 (A–E) and day 11 (F–J) of culture. Collagen type I (green) and propidium iodide staining (cell nuclei, red), untreated cpTi (A and F), cpTi modified by anodic oxidation at 10 V (B and G), 15 V (C and H), 20 V (D and I) and control glass (E and J). Objective 20×, magnification 2×, scale bar 20 μm.

Article Snippet: Saos-2 cells (passage 88, CLS Cell Lines Service GmbH, Germany) were seeded on cpTi scaffolds at the density 1.5 × 10 4 cells per cm −2 .

Techniques: Immunohistochemical staining, Staining, Cell Culture, Modification, Control

Journal: RSC Advances

Article Title: Different diameters of titanium dioxide nanotubes modulate Saos-2 osteoblast-like cell adhesion and osteogenic differentiation and nanomechanical properties of the surface †

doi: 10.1039/c9ra00761j

Figure Lengend Snippet: Immunohistochemical staining of osteocalcin (green) and propidium iodide staining (cell nuclei, red) in human osteosarcoma cells Saos-2 cultured on untreated cpTi (A), cpTi modified by anodic oxidation at 10 V (B), 15 V (C), 20 V (D) and control glass (E) on day 11. Objective 40×, magnification 1×, scale bar 20 μm, immerse oil.

Article Snippet: Saos-2 cells (passage 88, CLS Cell Lines Service GmbH, Germany) were seeded on cpTi scaffolds at the density 1.5 × 10 4 cells per cm −2 .

Techniques: Immunohistochemical staining, Staining, Cell Culture, Modification, Control

Journal: PLoS ONE

Article Title: The Coordinated P53 and Estrogen Receptor Cis-Regulation at an FLT1 Promoter SNP Is Specific to Genotoxic Stress and Estrogenic Compound

doi: 10.1371/journal.pone.0010236

Figure Lengend Snippet: To avoid any contribution of the endogenous p53 protein, gene reporter assays were performed in a p53-null, osteosarcoma-derived SaOS2 cell line. (A) Cells were transiently co-transfected with the FLT1-C or FLT1-T reporter constructs along with an overexpression vector for p53, p73β or p63γ as well as the pRL-SV40 control vector, followed by doxorubicin (0.3 µg/ml) or mock treatment, according to the schedule described in . Presented are the average-fold luciferase induction relative to FLT1-C mock treated and the standard errors of three replicates. Statistically significant differences relating to the impact of p53 expression alone or in combination with doxorubicin treatment are highlighted (* = p<0.01; ∧ = p<0.05, t-test). (B) The PG13 p53 reporter plasmid was used as a control for comparing the transactivation potential of p53, p73β or p63γ and the effect of the doxorubicin treatment.

Article Snippet: The metastatic neuroblastoma GIMEN cells (p53 wild type) were obtained from GP Tonini (National Institute for Cancer Research, IST, Genoa, Italy), while the colon adenocarcinoma HCT116 (p53 +/+ ) cell line and its p53 −/− derivative and the osteosarcoma-derived SaOS2 cells (p53-null) were a gift from B. Vogelstein (The Johns Hopkins Kimmel Cancer Center, Baltimore, Maryland, USA).

Techniques: Derivative Assay, Transfection, Construct, Over Expression, Plasmid Preparation, Control, Luciferase, Expressing